Escherichia coil,Saccharomyces cerevisiae, rat and human 3-methyladenine DNA glycosylases repair 1,N6-ethenoadenine when present in DNA
نویسندگان
چکیده
منابع مشابه
3-methyladenine DNA glycosylases: structure, function, and biological importance.
The genome continuously suffers damage due to its reactivity with chemical and physical agents. Finding such damage in genomes (that can be several million to several billion nucleotide base pairs in size) is a seemingly daunting task. 3-Methyladenine DNA glycosylases can initiate the base excision repair (BER) of an extraordinarily wide range of substrate bases. The advantage of such broad sub...
متن کاملHuman DNA Glycosylases Involved in the Repair
DNA molecules in cells constantly suffer from chemical decay due to exposure to endogenous and environmental agents, the immediate consequence of which is alterations to hydrogen bonding and base-stacking interactions in the DNA helix. Considering that such interactions are pivotal to the faithful expression and replication of genetic information, unrepaired DNA damage poses a substantial threa...
متن کاملThree-Dimensional Structure of a DNA Repair Enzyme, 3-Methyladenine DNA Glycosylase II, from Escherichia coli
The three-dimensional structure of Escherichia coli 3-methyladenine DNA glycosylase II, which removes numerous alkylated bases from DNA, was solved at 2.3 A resolution. The enzyme consists of three domains: one alpha + beta fold domain with a similarity to one-half of the eukaryotic TATA box-binding protein, and two all alpha-helical domains similar to those of Escherichia coli endonuclease III...
متن کاملInterplay between base excision repair activity and toxicity of 3-methyladenine DNA glycosylases in an E. coli complementation system.
DNA glycosylases carry out the first step of base excision repair by removing damaged bases from DNA. The N3-methyladenine (3MeA) DNA glycosylases specialize in alkylation repair and are either constitutively expressed or induced by exposure to alkylating agents. To study the functional and evolutionary significance of constitutive versus inducible expression, we expressed two closely related y...
متن کاملProperties and mechanism of action of eukaryotic 3-methyladenine-DNA glycosylases.
3-Methyladenine-DNA glycosylase activities have been identified in all eukaryotic cell systems studied. Some of the results from these studies are reviewed here. The enzymes possess molecular weights between 24 X 10(3) and 34 X 10(3), they have a broad pH optimum at approximately pH 8, require double-stranded DNA and act in the absence of any cofactors. The enzyme can excise several different m...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1995
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/23.18.3750